Enzyme Activity Measurement for Tyrosinase

Creative Enzymes is a leading company that provides high-quality bioanalytical services and contract-based research in enzyme activity analysis, serving the needs of our clients from the biotechnological and diagnostic industries. Equipped with the most advanced spectrophotometric instruments, we ensure that the test results are returned to our customers in a professional and timely manner. Here, we are proud to offer the most reliable activity measurement for tyrosinase.

Tyrosinase (EC 1.14.18.1; L-tyrosine,L-dopa:oxygen oxidoreductase) is a member of the type III copper protein family harboring a catalytic center formed by dinuclear copper, which is able to perform two successive reactions in the presence of molecular oxygen: the hydroxylation of phenols to form ortho-diphenols (monophenolase activity), and the oxidation of o-diphenols to o-quinones (diphenolase activity). Tyrosinase is mainly used for melanin formation, which is responsible for skin pigmentation and the browning of fruit and vegetables. The enzyme can also serve as a component in wound healing and primary immune response. Tyrosinase is ubiquitous in almost all domains of life where it is involved in various biological functions and the active site of this enzyme is well conserved among the different species. The active site is typically formed of six conserved histidine residues which coordinate with two copper ions, denoted CuA and CuB.

Tyrosinase has great potential for the production of various o-diphenols (also known as substituted catechols), which are important intermediates for the synthesis of agrochemicals, flavors, polymerization inhibitors, and antioxidants. In mammals, this enzyme is related to the skin pigmentation abnormalities, such as flecks and albinism. Therefore, the development and screening of potent inhibitors of tyrosinase are of particular interest to the cosmetics industry. In food industry, the inhibition of tyrosinase is routinely performed to avoid the oxidation of phenolic compounds, thus preventing the fruits from an undesirable taste and color. Additionally, tyrosinase have applications in wound healing, parasite encapsulation and insecticides. In general, tyrosinase is being a considerably attractive enzyme in multiple aspects. However, The dual activity owned by tyrosinase makes its activity assay sophisticated, especially on an industrial scale. Fortunately, Creative Enzymes has sufficient ability to establish the activity assay for tyrosinase, which is performed at 25°C by following the absorbance change at 280 nm using the spectrophotometric instrument. Creative Enzymes is your trustworthy partner during the development of any products containing tyrosinase. Our passion for high quality services and products is only exceeded by our excellent reputation in the global marketplace.

Figure: The crystal structure of tyrosinase from Bacillus megaterium.
PDB: 3NM8