Creative Enzymes is specialized in activity testing for hydrolases. We have the most cutting-edge instruments and a group of knowledgeable experts. The service quality is beyond the average level of the industry. The assay services for pancreatic ribonuclease are secured with reliable quantification.

Ribonucleases (RNases) are a group of enzymes with diverse structures and functions. These enzymes have general and specific activities. In prokaryotic and eukaryotic cells, there are two RNases, exo-RNases and endo-RNases respectively. They catalyze RNA cleavage reactions and have vital roles in RNA maturation and degradation, antiviral defense, and gene silencing. Pancreatic ribonuclease (EC 3.1.27.5) belongs to the endoribonucleases and catalyzes the cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.

In addition to their general function, pancreatic ribonuclease shows cytotoxicity toward tumor cell lines. This unusual biological activity is critically dependent on the ribonucleolytic activity and on the ability of these molecules to reach the cellular cytosol and to degrade RNA by evading the action of the ribonuclease inhibitor. Among these enzymes, bovine pancreatic ribonuclease is the best-studied member of the family and has served as a model system. The clinical therapy functions are the key research areas. Studies have shown that the dimeric enzyme proves to be a more efficient antitumor agent both in vitro and in vivo. Considering the applications in pharmacology and drug development, this enzyme from human sources has attracted a lot of interests. To facilitate such effort, Creative Enzymes provides customized activity assay services for pancreatic ribonuclease with guaranteed high-quality and quick turn-around time.

Figure: The crystal structure of pancreatic ribonuclease from Bos Taurus.
Reference: Santoro, J. et al. J.Mol.Biol. 1993 229: 722-734.

Creative Enzymes is equipped with sophisticated instruments and the superb team of scientists. We always persist in the best quality of activity determination. The excellent service in Creative Enzymes can satisfy your demands for any research purpose. In the future, we will continue updating facilitates and expertise to bring you perfect services.