Enzymes can catalyze substrate metabolism and play an important role in the operation of biological systems. As an important enzyme, oxidoreductase can help transfer electrons from donors to acceptors and promote redox reactions. Among oxidoreductases, we offer EC 1.3.99 enzymes with other acceptors enzyme activity assays. These enzymes specifically act on CH-CH groups and utilize a variety of acceptors to facilitate the transfer of electrons and protons.

Creative Enzymes has been the expert in enzyme activity assays for years. Using the resources of the most advanced spectrophotometers and a strong team of enzymologists, we have developed specific measurement methods for the oxidoreductase enzymes. We play an important role in helping researchers understand metabolic processes and their impact on chemical and biological studies and understand enzyme action and underlying mechanisms.

What do We Offer for Enzyme Activity Measurement?

In the case of EC 1.3.99 Enzyme Activity Assays with Other Receptors, we utilize spectrophotometry to obtain accurate measurements of enzyme activity. The spectrophotometric method we offer has been widely used in enzyme activity assays and has proven to be highly reliable.

We will prepare a specific assay volume mixture to measure the activity of an EC 1.3.99 enzyme, we select a substrate that usually contains a CH-CH group that can be oxidized. We then combine the substrate with the enzyme and a suitable acceptor molecule. Once the assay mixture is prepared, our researchers can add the final activation components to the mixture to initiate the activity assay. After the reaction materials are added to the observation vessel, all components should be mixed quickly and thoroughly at the beginning of the reaction. Data recording should be started immediately after mixing and plotted based on the detection signal over the complete time course of the reaction. Finally, we can determine the enzymatic activity of EC 1.3.99 versus other receptors by monitoring changes in absorbance over time.

The spectrophotometric method we provide mainly uses the difference in light absorption between the substrate and the product in the ultraviolet or visible part, selects an appropriate wavelength, and measures the progress of the reaction during the reaction. If a substrate or product can selectively absorb The activity of the enzyme can be judged based on the change in the absorbance value of the substance at a specific wavelength of light. Spectrophotometry has the characteristics of simple, fast and accurate application in EC 1.3.99 enzyme determination.

Role of EC 1.3.99 With Other Acceptors in Metabolism and Catabolism

EC 1.3.99 enzymes with other acceptors play a crucial role in the metabolism and catabolism of various organic compounds. These enzymes participate in essential pathways involved in the breakdown of complex molecules, allowing organisms to extract energy and generate metabolic intermediates. One important process in which EC 1.3.99 enzymes function is the degradation of fatty acids. These enzymes facilitate the conversion of fatty acids into acetyl-CoA units, which are subsequently able to enter the citric acid cycle and supply additional energy. Furthermore, various acceptors are engaged in the metabolism of amino acids by EC 1.3.99 enzymes, the creation of critical components like neurotransmitters and nucleotides is aided by the conversion of these amino acids, in addition to aiding in the production of energy.

Pathways Involving EC 1.3.99 With Other Acceptors

• Beta-Oxidation of Fatty Acids. EC 1.3.99 enzymes act on the CH-CH groups of fatty acids, catalyzing their oxidation to generate acetyl-CoA units.
• Amino Acid Degradation. Keto acids are created when specific amino acids undergo deamination. As part of the breakdown process, EC 1.3.99 enzymes may target the CH-CH groups inside these keto acids.
• Alkene Metabolism. Alkenes like unsaturated fatty acids are metabolized by enzymes with the EC 1.3.99 class. These enzymes aid in the breakdown and subsequent use of these chemicals by specifically targeting the CH-CH groups that are present in them.

Our service advantages

Creative Enzymes is dedicated to the field of enzyme research, and we offer EC 1.3.99 with other receptor enzymes assays, play a crucial role in metabolism and catabolism chemically. Using spectrophotometry, we can accurately measure their enzymatic activity, providing valuable insights into biological processes. If you are interested in us, please feel free to contact us.