Enzymes are biocatalysts that play a vital role in biological reactions by accelerating the rate of these reactions. Among the numerous enzyme classes, EC 1.3.7 falls under a specific group known as oxidoreductases. EC 1.3.7 enzymes specifically act on CH-CH groups, transferring electrons from a donor molecule to an acceptor molecule. Iron-sulfur proteins are important components in various biological processes, including electron transfer chains and enzymatic reactions. EC 1.3.7 enzymes catalyze reactions where the transfer of electrons to an iron-sulfur protein is an essential step.

Creative Enzymes is an expert in enzyme activity measurement. Based on our rich experience and advanced technology, we provide high-quality and accurate enzyme activity measurement services. Our strict quality control system ensures high precision and small variation of test results, especially for oxidoreductases including with an iron-sulfur protein as acceptor.

Determination of Enzyme Activity

In the determination of enzyme activity for reactions such as EC 1.3.7, we utilize a spectrophotometric assay to obtain an accurate determination of enzyme activity by measuring light absorption at specific wavelengths. For the determination of this type of enzyme activity, we need to prepare a reaction mixture containing the enzyme, substrate and iron-sulfur protein receptor. As the reaction proceeds, the enzyme catalyzes the transfer of electrons from the substrate to the iron-sulfur protein. This transfer results in a change in absorbance at a specific wavelength, which allows the enzyme activity to be quantified by spectrophotometric analysis. Our use of spectrophotometry provides an effective method for the accurate determination of enzyme activity, by which we can help researchers accurately measure the rate of electron transfer and enzyme activity, providing valuable data for further biological research and analysis.

The Role and Pathways of EC 1.3.7 With an Iron-Sulfur Protein as Acceptor

EC 1.3.7 Enzymes play a vital role in biological systems. By catalyzing the transfer of electrons from donor molecules to iron-sulfur protein acceptors, these enzymes are involved in various physiological pathways.EC 1.3.7 An important pathway in which enzymes are involved is the electron transport chain. In this process, electrons produced by cellular respiration are sequentially transferred through electron carriers, including iron-sulfur proteins. Enzymes in which iron-sulfur proteins act as electron acceptors contain non-heme iron and acid-unstable sulfur, which transfer electrons with changes in the iron valence state. Iron-sulfur proteins besides being electron transferors in the respiratory combined photosynthetic chain also act as cofactors for several enzymes and are involved in catalytic reactions, as well as in the biosynthesis of several important biomolecules. These enzymes play a key role in the construction and modification of complex molecules by transferring electrons from CH-CH groups to iron-sulfur proteins.

Our Service Workflow

Creative Enzymes provides reliable technical support for iron-sulfur receptor-based enzyme activity analysis based on advanced technology platforms and experienced research teams. Our enzyme activity analysis has currently provided valuable data support for a wide range of research fields, such as biology, medicine, environmental science, etc. If you are interested in us, please feel free to contact us.