Enzyme Activity Measurement for Oxidoreductases Acting on CH-CH Groups With an FAD as Prosthetic Group

EC 1.3.8 with FAD as a prosthetic group, represents a group of oxidoreductases that are responsible for catalyzing reactions involving CH-CH groups. These enzymes act as essential biocatalysts, facilitating the transfer of electrons within metabolic pathways. These enzymes can mediate the oxidation or reduction of substrates containing CH-CH groups because the FAD functions as both an electron donor and acceptor during redox processes. Creative Enzymes is an industry leader in the field of enzyme assays, and our spectrophotometric assays enable researchers to accurately measure the activity of EC 1.3.8 enzymes and gain a better understanding of their biochemical properties, substrate specificity, and regulatory mechanisms.

What do We Offer for Enzyme Activity Measurement?

We utilized a spectrophotometric assay that was used to accurately measure the enzymatic activity of EC 1.3.8 with an FAD as prosthetic group. In a spectrophotometric assay, the reaction rate is quantified by utilizing the interaction between light and the enzyme reaction.

Our process is as follows:

• Sample Preparation. Before initiating the assay, the enzyme needs to be purified or isolated from a biological source. The pure enzyme provides more accurate and reliable results as it eliminates potential interfering factors.
• Preparation of Reaction Mixture. The reaction mixture is prepared by combining the purified EC 1.3.8 enzyme, the substrate carrying CH-CH groups, necessary co-factors (if any), and buffers to maintain optimal pH and conditions for enzyme stability and activity. Additionally, FAD is included as a prosthetic group essential for the enzymatic reaction.
• Incubation and Monitoring. The reaction mixture is incubated under controlled conditions to allow the enzymatic reaction to occur. During this incubation period, the formation of intermediates and products can be monitored using the spectrophotometry technique. These techniques enable the quantification of the enzymatic activity by measuring changes in absorbance or fluorescence intensity, corresponding to the progress of the reaction.
• Data Analysis and Enzyme Activity Calculation. Based on the obtained measurements, our researchers can determine the initial reaction rate, enzymatic turnover, and other kinetic parameters. By comparing with controls and standard curves obtain the enzyme activity.

Determine the significance of EC 1.3.8 with FAD as a Prosthetic Group

• Cellular Regulation. EC 1.3.8 enzymes with FAD as a prosthetic group are often regulated to maintain proper metabolic balance within cells. Regulation can occur through various mechanisms, including allosteric regulation, post-translational modifications, and gene expression control. Understanding the regulation of these enzymes is crucial for comprehending their roles in physiological processes and disease states.
• Biotechnological Applications. EC 1.3.8 enzymes with FAD as a prosthetic group find applications in different biotechnological fields. Comprehending the enzymatic activity of these oxidoreductases, for example, can help in the development of enhanced biocatalysts for industrial processes, including the manufacture of biofuels, fine chemicals, and medicinal substances. These enzymes can also be used to generate new biosynthetic pathways through metabolic engineering.
• Medical Implications. Dysregulation or mutations in EC 1.3.8 enzymes with FAD as a prosthetic group can lead to various diseases. For instance, metabolic diseases may arise from deficits in particular enzymes involved in the oxidation of fatty acids. Researching these enzymes' functions and activities can aid in the diagnosis of various illnesses and provide light on their underlying causes, which will enhance clinical care and the development of new therapeutic approaches.

Our service workflow

Creative Enzymes is dedicated to enzyme activity detection. Based on our advanced technology platform and experienced team of enzymology experts, we have developed specific methods for enzyme activity determination. Our enzyme activity assay services support the precise determination of enzyme activity, greatly facilitating enzyme research. If you are interested in us, please feel free to contact us.