Enzyme Activity Measurement for Non-Specific Serine/Threonine Protein Kinase

Creative Enzymes has been active in the industry of enzyme activity assays for many years. With continuous efforts on discovering and developing new assays methods, Creative Enzymes accumulated extensive experiences in the field of enzyme activity quantification, and has earned the trust of many customers. We’re able to work together with our customers through the service period of solving specific problems, optimizing processes, and developing new enzymatic assays. Herein, Creative Enzymes is glad to offer the reliable enzyme activity measurement for non-specific serine/threonine protein kinases.

Non-specific serine/threonine protein kinases (EC 2.7.11.1; ATP:protein phosphotransferase (non-specific)) are a heterogeneous group of serine/threonine protein kinases that phosphorylate the OH group of a serine or threonine sidechain in proteins. The phosphorylation of a protein plays a pivotal role in various cellular processes and in posttranslational modification. EC 2.7.11.1 describes a sort of enzymes that belong to transferases, specifically protein-serine/threonine kinases. These enzymes are not designated with individual EC numbers and were formerly categorized under EC 2.7.1.37, which was a general EC number for any enzyme that phosphorylates protein while converting ATP to ADP.

Functionally, non-specific serine/threonine protein kinases play an important role in the modulation of cell proliferation, cell apoptosis, cell differentiation, and embryonic development. In addition, it has been reported that non-specific serine/threonine protein kinases are related to many types of cancer. Ribosomal S6 kinase (RSK) was shown to be involved in the development of some prostate cancers.

Because of the diversity of the class of non-specific serine/threonine protein kinases, the enzyme activity assays for these miscellaneous enzymes appear to be difficult and challenging. Nonetheless, Creative Enzymes has been prepared for proper enzyme assays for these enzymes. We have a group of superb scientists who spent most of their careers on discovering and developing various methods of enzyme activity assays. The highest quality of services is one of the pillars of our global success and the basis of our high-level customer satisfaction. Overall, Creative Enzymes will be your best choice for the first-class technical support and problem solving source.

Figure: The crystal structure of human Aurora-A protein kinase.
PDB: 1MQ4