Enzyme Activity Measurement for Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)

Creative Enzymes is well known to provide the most efficient research services, as well as custom assays. We have accumulated extensive experiences on enzyme activity measurement over the past decade. The professional processes and high service quality of Creative Enzymes support researchers and innovators in various industries for testing oxidoreductases, including glyceraldehyde-3-phosphate dehydrogenase (phosphorylating).

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (EC 1.2.1.12) is a tetrameric enzyme and located in the cytoplasm, which plays a pivotal role in the Embden-Meyerhoff pathway not only in glycolysis but also in glyconeogenesis. This enzyme catalyzes the reversible oxidative phosphorylation of D-glyceraldehyde 3-phosphate (G3P) into 1,3-bisphosphoglycerate (1,3-bisPGA) using the NAD⁺ as the cofactor. The enzyme has been studied extensively from animals and microorganisms and shown to have properties that are likely to be important in regulating the flux through the glycolytic pathway. This enzyme is a member of the oxidoreductases family, and the systematic name of this enzyme class is D-glyceraldehyde-3-phosphate:NAD⁺ oxidoreductase (phosphorylating), which is also known as:

• triosephosphate dehydrogenase;
• dehydrogenase, glyceraldehyde phosphate;
• phosphoglyceraldehyde dehydrogenase;
• 3-phosphoglyceraldehyde dehydrogenase;
• NAD⁺-dependent glyceraldehyde phosphate dehydrogenase;
• glyceraldehyde phosphate dehydrogenase (NAD⁺);
• glyceraldehyde-3-phosphate dehydrogenase (NAD⁺);
• NADH-glyceraldehyde phosphate dehydrogenase;
• glyceraldehyde-3-P-dehydrogenase

Figure: The crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Sulfolobus solfataricus.
PDB: 1B7G

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) is a vital enzyme in various pathways, including glycolysis/gluconeogenesis pathways, carbon fixation in photosynthetic organisms, metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics and microbial metabolism in diverse environments. It should be emphasized that this enzyme plays an absolutely important role in glycolysis/gluconeogenesis pathways. Gluconeogenesis is the generation of glucose from non-sugar carbon substrates such as pyruvate, (S)-lactate , glycerol , and glucogenic amino acids.

In addition, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) also serves as a critical role in certain neurological disorders, such as Alzheimer’s disease, Parkinson’s disease and Huntington’s disease. To conclude, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) has potential applications in biotechnology, biochemistry, medical and pharmaceutical industries due to its central functions in multiple biological processes. As an increasing number of studies on the enzyme have been initiated, proper and efficient activity assays are much desired. Creative Enzymes has displayed superb technologies, professional knowledge, and the most advanced equipment, which make our enzyme activity measurement of the utmost quality, especially for glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). Our prompt service, good customer care, and dedicated approach has made us the most preferred service provider.