Enzyme Activity Measurement for Glucuronoarabinoxylan Endo-1,4-Beta-Xylanase

Creative Enzymes is proud to offer various enzyme activity assays for customers, such as rapid routine quantification and custom activity tests. We are committed to being the most reliable supplier for enzyme assays in the global market. Our prompt service, best customer care and dedicated approaches have made us the most preferred provider. Having tested numerous enzymes in the past several years, Creative Enzymes has accumulated extensive research experiences, which is the basis to provide rapid and high-quality enzyme activity assays for glucuronoarabinoxylan endo-1,4-beta-xylanase.

Glucuronoarabinoxylan endo-1,4-beta-xylanase (EC 3.2.1.136; glucuronoarabinoxylan 4-beta-D-xylanohydrolase) is an enzyme that catalyzes the endo-hydrolysis of (1,4)-β-D-xylosyl links in some glucuronoarabinoxylans. In addition, this enzyme presents a high activity towards feruloylated arabinoxylans from cereal plant cell walls. Recent studies have shown that glucuronoarabinoxylan endo-1,4-beta-xylanase from the Gram-positive bacterium Bacillus subtilis is from glycosyl hydrolases (GH) family 5. The major interest on this enzyme is the specificity and mode of action on the complex substrate glucuronoxylan and the characterization of the xylooligomeric products generated. The enzyme is glucuronoxylan-specific and cleaves the β-1,4-linked main xylan chain in an endo manner directed by the position of an α-1,2-linked glucuronate moiety. Besides, many reports have made a conclusion that cleavage happens at the second β-1,4 linkage toward the reducing terminus from a glucuronate-substituted xylose. This mode of action leads to signally substituted glucurono-xylooligosaccharides with the substitution penultimate to the reducing terminus. Functionally, glucuronoarabinoxylan endo-1,4-beta-xylanase serves as a critical role in the pre-treatment for ethanol formation from lignin-cellulose fibers. Furthermore, this enzyme can also be exploited in the food industry, such as in the fermentation and storing.

Because the enzyme is relatively newly discovered and identified, little research has been done to understand the properties of the enzyme. Properly measuring its activity is even more difficult without the above-mentioned information. However, Creative Enzymes relies on its own expert team and develops robust activity analysis to satisfy customer’s request. The activity of glucuronoarabinoxylan endo-1,4-beta-xylanase is determined by spectrophotometric assays. The test results are the most trusted and proven, which are assured by our state-of-the-art technology and the most advanced instrument. Overall, Creative Enzymes is your best choice for enzyme activity assays and will always provide the best customer satisfaction.

Figure: The crystal structure of the glucuronoxylan xylanohydrolase XynC from B. subtilis.
PDB: 3KL0