Enzyme Activity Measurement for Glucan 1,4-Alpha-Glucosidase

Creative Enzymes has more than ten years of experiences in enzyme activity measurement. Our high-quality services can meet the requirements for both academia and industry. With the advanced equipment in the laboratory center, the activity measurement for glucan 1,4-alpha-glucosidase is performed by our professional experts.

Glucan 1,4-alpha-glucosidases (glucoamylases, EC 3.2.1.3) exist in a variety of organisms. These enzymes are exo-glycosyl hydrolases which catalyze the release of β-glucose from the non-reducing ends of starch and other related poly and oligosaccharides. In addition, glucoamylase is able to degrade α-1,6-glucisidic linkages, although much less effectively.

Glycosyl hydrolases have been classified into more than 60 families on the basis of sequence similarity. Glucoamylases belong to family 15 and have been well characterized biochemically, mainly through studies of fungal and yeast enzymes. The primary industrial use of glucoamylase is in the production of glucose and fructose syrups, which in turn serve as a feedstock for biological fermentations in the production of ethanol or high fructose sweeteners. Besides, it holds second position after proteases in the worldwide distribution and sales of industrial enzymes. The clinical studies indicate that the enzyme inhibitors are very effective in controlling blood glucose levels, and thus are lead candidates for the treatment of type 2 diabetes. The activity measurement of glucoamylases is done by detecting the increment of glucose concentration. The widely used Nelson-Somogyi method is based on the absorbance at 520 nm of a colored complex between a copper oxidized sugar and arsenomolybdate, using spectrophotometric assays. Creative Enzymes has strong abilities to perform accurate activity assays for glucan 1,4-alpha-glucosidases by the Nelson-Somogyi method.

Figure: The crystal structure of the complex of a glucan 1,4-alpha-glucosidase from Saccharomycopsis fibuligera with acarbose.
Reference: Sevcik, J. et al. Febs J. 2006 273: 2161-2171.

Creative Enzymes is the top service supplier for enzyme activity testing. Our advantages include the professional research team, the cutting-edge equipment, and the extensive expertise. Over the years, we have gained good reputations for the effective tests, rapid turn-around, and dedicate customer care. In the future, we have confidence to continue to be your trustworthy partner.