Enzyme Activity Measurement for Aldehyde Reductase Using Spectrophotometric Assays

Creative Enzymes is capable to provide reliable and rapid enzymatic activity measurement for aldehyde reductase. The activity assays are performed by spectrophotometric methods with the most advanced analytic instruments. Creative Enzymes is well known for the professional techniques and extensive experiences, ensuring the customers to obtain the precise and reproducible activity tests result for aldehyde reductase.

Aldehyde reductase is a monomeric NAD- or NADP-dependent enzyme (NAD: nicotinamide adenine dinucleotide; NADP: nicotinamide adenine dinucleotide phosphate). It is a member of aldo-keto reductase superfamily. The catalytic active site is located in a barrel core consisting of α-helices and β-sheets. The NADPH cofactor is situated at the top of the β/α barrel.Aldehyde reductase (or aldose reductase, EC 1.1.1.21) catalyzes the reduction of a variety of aldehydes and carbonyls, including monosaccharides. Aldehyde reductase is mainly known for catalyzing the reduction of glucose to sorbitol, which is the first step in the polyol pathway of glucose metabolism. The aldose reductase reaction is important for the function of various organs in the body, such as seminal vesicles, liver, lens, retina, Schwann cells of peripheral nerves, placenta, and red blood cells.

The reduction of glucose by the aldehyde reductase-catalyzed polyol pathway has been related to the secondary diabetic complications development. Recent studies recommend that glucose may be an incidental substrate of aldehyde reductase, which seems to be more adept in catalyzing the aldehydes reduction created by lipid peroxidation. Additionally, aldehyde reductase inhibition has been demonstrated to increase vascular oxidative stress induced by inflammation and also to prevent myocardial protection related to the late phase of ischemic preconditioning. Based on these studies, aldehyde reductase has been ascribed an important antioxidant role. Because of its significant in health care, the enzymatic activity measurement of aldehyde reductase has become an increasing need for researchers. Creative enzymes offers accurate quantification method to determine the aldehyde reductase activity by spectrophotometer and is your trustworthy partner for enzymes activity measurement.

Figure: Human aldose reductase in complex with NADP+, citrate, and IDD594, a small molecule inhibitor.

Reference: Howard EI, Sanishvili R, Cachau RE, et al (June 2004). "Ultrahigh resolution drug design I: details of interactions in human aldose reductase-inhibitor complex at 0.66 Å". Proteins 55 (4): 792–804.