Enzyme Activity Measurement for Aldehyde Dehydrogenase [NAD(P)+]

Creative Enzymes is a leader in the field of enzyme services who is committed to being the most reliable provider for enzyme activity assays in the global market. We are proud to offer the most precise activity assay for aldehyde dehydrogenase [NAD(P)⁺], which is backed by a team of extraordinary enzymologists.

Aldehyde dehydrogenases [NAD(P)⁺] (EC 1.2.1.5; ALDH) are a group of enzymes that catalyze the conversation of aldehydes to the corresponding carboxylic acids by means of an NAD(P)⁺-dependent virtually irreversible reaction. Aldehydes, the substrates of this reaction, are ubiquitous in nature and most of them are toxic at low levels because of high chemical reactivity. The level of the metabolic-intermediate aldehydes are normally regulated carefully by the body. In addition, compounds with aldehydic functional groups are generated as important intermediates in many catabolic and biosynthetic pathways. Moreover, the ALDH-mediated generation of NADH/NADPH represents a major source of reducing equivalents required for maintaining the cellular redox balance. This enzyme can be found in both prokaryotes and eukaryotes, such as Bacillus licheniformis, Burkholderia xenovorans, human, mouse, and bovine. The systematic name of this enzyme class is aldehyde:NAD(P)⁺ oxidoreductase. Note that aldehyde dehydrogenase [NAD(P)⁺] plays a crucial role in many catabolic and bio-synthetic pathways including:

• Glycolysis / Gluconeogenesis;
• Histidine metabolism;
• Tyrosine metabolism;
• Phenylalanine metabolism;
• beta-Alanine metabolism;
• Metabolism of xenobiotics by cytochrome P₄₅₀;
• Drug metabolism - cytochrome P₄₅₀;
• Metabolic pathways;
• Biosynthesis of secondary metabolites;
• Microbial metabolism in diverse environments.

The cytoprotective action of the enzymes during oxidative stress represents another key function of the aldehyde dehydrogenases. Under conditions inducing oxidative stress, aldehyde dehydrogenases serve as “aldehyde scavengers” via metabolizing reactive aldehydes produced as a consequence of the oxidative degradation of lipid membranes. It can be seen that aldehyde dehydrogenase [NAD(P)⁺] acts as the key enzyme in biotechnology, and thus more studies have been focused on this enzyme to understand the catalytic mechanism in details. Creative Enzymes is one of the few companies in the world that have the capability of providing spectrophotometric activity assays for aldehyde dehydrogenase [NAD(P)⁺]. We have the most advanced spectrophotometric instruments and the leading techniques, which assure high accuracy of the results and the best customer satisfaction. Owning the best customer care, the prompt service, and the spirit of constantly pursuing utmost quality service, Creative Enzymes is looking forward to establish a long-term relationship with all customers.

Figure: The crystal structure of the NADP+-dependent aldehyde dehydrogenase from Vibrio harveyi.
PDB: 1EYY