Enzyme Activity Measurement for 6-Phospho-Beta-Glucosidase

With decades of experiences and unique technologies, Creative Enzymes distinguishes itself with the commitment to providing enzyme activity assays of the highest quality to customers in academia and industry. We partner with diverse and numerous customers to support their needs in exploratory research and process optimization. The unique advantage of our spectrophotometric analysis allows us to surpass competitors and assures the reliability of the test results. Herein, Creative Enzymes is proud to offer the accurate enzymatic activity measurement for 6-phospho-beta-glucosidase.

6-Phospho-beta-glucosidase (EC 3.2.1.86; 6-phospho-beta-D-glucosyl-(1,4)-D-glucose glucohydrolase) belongs to glycoside hydrolase family 1 (GH1) and catalyzes the hydrolysis of β-1,4-linked cellobiose 6-phosphate (cellobiose-6’P) to yield glucose and glucose 6-phosphate, which is an important cellular precursor. The products of this reaction are further metabolized by the energy-generating glycolytic pathway. 6-Phospho-beta-glucosidase is a single-domain protein that adopts a (β/α)₈-barrel structure, which is a typical fold of GH1 family members. According to the CAZy database, the GH1 family shows quite diverse enzyme functions and is composed of hydrolases with 19 enzymatic. 6-Phospho-beta-glucosidase from Thermotoga maritima is composed of a catalytic module of 415 amino acid residues and most likely exists in a dimer-tetramer equilibrium in solution. 6-Phospho-beta-glucosidase from T. maritima requires both NAD⁺ and Mn²⁺ for catalysis.

Figure 1: Proposed mechanism of 6-phospho-beta-glucosidase from T. maritima.
Reference: Vivian L. Y. Yip, Annabelle Varrot, Gideon J. Davies, et al. Journal of the American Chemical Society, 2004, 126(27):8354.

Creative Enzymes provides the most accurate enzymatic activity assay for 6-phospho-beta-glucosidase. For details, the enzyme activity is determined in a 6-phospho-beta-glucosidase/glucose-6-P-dehydrogenase (G6PDH) coupled reaction, where oxidation of 6-P-β-glucose is coupled to the reduction of NADP⁺, and the increased absorbance at 340 nm is measured. In fact, the results of our test are the most trusted and proven, which is assured by the most advanced spectrophotometric instruments and our state-of-the-art technology. Besides, our scientists always focus on discovering detailed properties of enzymes and optimizing the assay methods. Overall, Creative Enzymes is your trusty-worthy partner and will deliver the first-class customer satisfaction.

Figure 2: The crystal structure of 6-phospho-beta-glucosidase from T. maritima.
PDB: 1UP4