Creative Enzymes provides most precise activity measurement for 17 beta-hydroxysteroid dehydrogenase to the customers. We are a group of scientists who have been working on enzyme activity assays for years. The unique advantage on our spectrophotometric analysis allows us to outpace our competitors and assures the reliability of the test results.

17 Beta-hydroxysteroid dehydrogenase (EC1.1.1.51) is an NAD(H) and/or NADP(H) dependent enzyme that catalyzes the dehydrogenation of 17-hydroxysteroids in steroidogenesis. This reaction includes interconversion of DHEA (Dehydroepiandrosterone) and androstenediol, androstenedione and testosterone, and estrone and estradiol, respectively. It is worth noting that this enzyme plays a valuable role in many pathways:

• Steroid hormone biosynthesis;
• Steroid degradation;
• Metabolic pathways;
• Microbial metabolism in diverse environments

For many reactions that the enzyme catalyzes, it could also go with other names:

• beta-hydroxy steroid dehydrogenase;
• 17-ketoreductase;
• 17beta-hydroxy steroid dehydrogenase;
• 17β-HSD;
• 3beta-hydroxysteroid dehydrogenase;
• 3beta-hydroxy steroid dehydrogenase;
• 17-ketosteroid oxidoreductases;
• 17-ketosteroid reductases;
• HSD17B;
• 17-KSR

One significant role of 17 beta-hydroxysteroid dehydrogenase lies in regulation of the biological activity of steroid hormones. The hormone is regulated at the pre-receptor level by several enzymes including 17 beta-hydroxysteroid dehydrogenase. The enzyme is present in many microorganisms, invertebrates and vertebrates. Dysfunctions in human 17 beta-hydroxysteroid dehydrogenase result in many disorders, including reproduction and neuronal diseases. The enzyme is also critical to the pathogenesis of miscellaneous cancers. 17 Beta-hydroxysteroid dehydrogenase reveals a remarkable multifunctionality being able to modulate concentrations not only of steroids but also of fatty and bile acids. It can be seen that 17 Beta-hydroxysteroid dehydrogenase has a significant meaning in many fields such as chemical and pharmaceutical industries and can play a valuable role in the treatment of many cancers. However, the measurement of activity assay for this enzyme is perplexing and it has not been well developed. Fortunately, Creative Enzymes made the accurate activity assay available for 17 Beta-hydroxysteroid dehydrogenase, based on years of experiences on testing the enzymes and optimizing the method. The spectrophotometric assay was demonstrated to be a reliable method for activity quantification the enzyme. The unique and superior service distinguishes Creative Enzymes from our competitors. Overall, Creative Enzymes is your best choice during development of products containing 17 Beta-hydroxysteroid dehydrogenase.

Figure: Structural organization of 3α/17α-HSD. Ribbon representation of the 3α/17α-HSD monomer.
Reference: Jordi Benach et al., Biochemistry. 2002, 41, 14659-14668.