Enzyme Discovery

Creative Enzymes occupies a unique position in the enzyme market, providing strategic solutions for finding promising industrial enzymes. We can provide enzyme screening services from natural sources such as soil microorganisms, plants, and animals, as well as enzyme gene screening services based on databases. Creative Enzymes combines advanced biotechnology, including separation and isolation technology, high-throughput screening technology, bioinformatics tools, metagenomic libraries, etc., to provide a full set of enzyme discovery services from initial sample preparation to final identification of novel enzymes.

Figure 1. Main Strategies for Novel Enzymes Discovery
(Jacques, Philippe, et al. Bioprocess and Biosystems Engineering 2017)

Enzyme Screening Services

• Enzyme Screening from Natural Sources

Enzymes have found applications as biocatalysts with high efficiency and selectivity in manifold fields of chemistry and biology. However, only a small proportion of natural enzymes have been exploited industrially. Screening enzyme-producing microorganisms from nature is a traditional strategy for discovering novel enzymes. The process includes sample collection, enrichment culture, strain isolation and purification, primary and secondary screening, and verification, which is usually very time-consuming and has limited sensitivity. Creative Enzymes has built a professional high-throughput screening platform for enzyme discovery, which can screen target enzymes more accurately, sensitively, and quickly.

• Enzyme Screening Based on Genome Mining

Directly mining enzymes with required functions from genetic databases is a more direct and effective screening method. The molecular screening method is to obtain candidate enzyme genes by searching and analyzing the sequenced entire genome of microorganisms. Sequence-based screening methods use known enzymes as probes to obtain enzyme genes with moderate similarity in database. Then the enzyme gene is cloned and expressed. The candidate enzyme is obtained through functional screening.

Strategies for enzyme discovery can be divided into two pathways, which can be combined to obtain optimal results. The first involves direct detection of the desired enzyme activity. Based on next-generation sequencing (NGS) methods, Creative Enzymes constructs metagenomic libraries and develops high-throughput screening for enzymatic activity. The second strategy involves functional prediction and in silico screening. Creative Enzymes applies genome mining approaches for enzyme discovery based on similarities of their DNA sequences or 3D structures to those of known activity. We also investigate interactions among different enzymes in case a series of enzymes need to stay in the system for the best performance.

Enzyme Synthesis Services

Enzyme synthesis is a critical field in biotechnology, and it has led to the development of innovative solutions for numerous industries. Custom-designed enzymes can provide more efficient and sustainable methods for various processes, making them an essential tool in modern biotechnology and industrial applications.

Creative Enzymes is a leading provider of enzyme synthesis services that cater to a wide range of research, industrial, and pharmaceutical needs. With our cutting-edge expertise in enzyme technology and biocatalysis, we offer tailored solutions for the efficient production of enzymes with precise specifications. Key features of our enzyme synthesis services include customization, high quality, scale-up capabilities, timely delivery, comprehensive support, and competitive pricing.

Enzyme Structure Characterization Services

Creative Enzymes conducts structure and activity characterization for the identification of novel enzymes. We offer services on demand, including primary structure determination, disulfide bond analysis, and 3D structure determination. Our enzyme structure characterization services encompass a wide range of cutting-edge techniques and approaches, including but not limited to X-ray Crystallography, NMR Spectroscopy, Cryo-Electron Microscopy (Cryo-EM), Mass Spectrometry, Homology Modeling, etc. By utilizing our Enzyme Structure Characterization Services, researchers and organizations gain a deeper understanding of enzyme functionality, which can be applied to drug design, enzyme engineering, and a wide array of biotechnological and pharmaceutical applications.

Enzyme discovery represents a growing field with many possible applications including animal feed, biocatalysts, pharmaceuticals, and chemical processing. Creative Enzymes has tight connections in both academia and industry and thus has accumulated rich experience to provide the most advanced solutions for our customers.

Figure 2. Enzyme Discovery Workflow