Enzyme Activity Measurement for Spleen Exonuclease

Creative Enzymes is an industrial biotech company specialized in enzyme activity assays. We provide our customers with highly customized services of enzymatic analysis and serve the needs of our countless clients from the pharmaceutical, biotechnological and diagnostic industries. Herein, Creative Enzymes is proud to present the most precise enzyme activity quantitation for spleen exonuclease.

Spleen exonuclease (EC 3.1.16.1; formerly EC 3.1.4.18) is a phosphodiesterase sequentially splits off nucleoside 3’-phosphates from the 5’-end of both RNA and DNA. This kind of enzyme activity is related with various essential cell processes such as DNA repair and replication, genetic recombination, apoptosis, and degradation of unnecessary and redundant nucleic acids and oligonucleotides. Spleen exonuclease is able to digest mRNA, RNA core, tRNA, poly(A), poly(U), and alkali-denatured DNA, whereas poly(C), poly(T), and native DNA resist hydrolysis. Many studies suggest that the base specificity of this enzyme plays a key role in the structure-activity relationship.

Spleen exonuclease can be found in eukaryotes and archaea, such as human, pig, Trypanosoma brucei, and Sulfolobus tokodaii. Although this enzyme belongs to the group of long-time discovered nucleotidic enzymes, the knowledge on its properties, particularly on its structural requirements is very limited. The present knowledge on spleen exonuclease may be summarized as follows: (1) The enzyme degrades specifically the poly- and oligonucleotides of both the ribo and deoxyribo series to 3' -nucleotides regardless the character of the heterocyclic base; (2) The enzyme possesses the typical character of an exonuclease and the degradation begins at the 5'-end of the oligonucleotide; (3) The enzyme is sensitive towards the secondary structure of the substrate. The mechanism of the action of spleen exonuclease has not been hitherto studied in detail, possibly because very few spleen exonucleases have been identified and characterized, and the activity measurement of this enzyme seems to be quite difficult. Fortunately, this situation is not a ticklish question for Creative Enzymes. In fact, Creative Enzymes is completely competent to offer the reliable enzyme activity assay for spleen exonuclease, due to our extensive research experience and the state-of-the-art technology. The activity of spleen exonuclease can be measured by using the spectrophotometric assays. The passion of Creative Enzymes for high quality and unique services is only exceeded by its excellent reputation in the marketplace. Overall, Creative Enzymes is your best choice which is well known to provide the best customer satisfaction.