Enzyme Activity Measurement for Oxidoreductases Acting on CH-CH2 Groups With MCD as Acceptor

EC 1.17.2 designates oxidoreductases acting on CH-CH2 groups with MCD (1-methylenecyclohex-1-ene-3-carboxylate) as an acceptor. These enzymes play a crucial role in various metabolic pathways, facilitating the transfer of electrons between substrates. The utilization of MCD as an acceptor molecule underscores its significance in redox reactions within biological systems.

Creative Enzymes has been the expert in enzyme activity assays for years. Using the resources of the most advanced spectrophotometers and a strong team of enzymologists, we have developed specific measurement methods for enzyme activity measurement.

What do We Offer for Enzyme Activity Measurement?

The spectrophotometric determination of EC 1.17.2 enzyme activity involves measuring changes in absorbance at specific wavelengths corresponding to the reaction products or intermediates. This method allows for the precise quantification of enzymatic activity by monitoring the rate of substrate conversion or product formation over time.

• Preparation of Assay Mixture. Combine the enzyme sample, substrate, cofactors, and MCD as an acceptor prepared in a suitable buffer under optimal conditions for enzyme activity.
• Baseline Measurement. Record the initial absorbance of the assay mixture at the appropriate wavelength.
• Initiation of Reaction. Add the substrate to the assay mixture and immediately initiate the reaction.
• Monitoring Absorbance Changes. Continuously measure the absorbance at specified time intervals to track the progression of the reaction.
• Calculation of Enzyme Activity. Determine the rate of change in absorbance over time and correlate it with the enzymatic activity using appropriate calibration curves or standard references.

Pathways Involving EC 1.17.2 With MCD as Acceptor

Enzymes belonging to EC 1.17.2 that use MCD as an acceptor are involved in many pathways that involve CH-CH2 group redox reactions. These enzymes break down long-chain fatty acids through several pathways, one of which is the fatty acid oxidation pathway. The removal of successive acetyl-CoA units from the fatty acid chain is catalyzed by EC 1.17.2 enzymes during fatty acid oxidation, producing reducing equivalents in the form of NADH or FADH2. The cell uses these reducing equivalents in addition to producing ATP, which is the unit of account for energy in cells.

Significance of Studying EC 1.17.2 With MCD as Acceptor

Accurate determination of EC 1.17.2 Enzymes have important application value in drug development and industrial biotechnology. For example, in the field of drug discovery, EC 1.17.2 enzymes serve as potential targets for drug design, particularly in diseases related to fatty acid metabolism and energy homeostasis, by helping researchers elucidate the structure-function relationships of these enzymes. One strategy to treat metabolic diseases is to design particular activators or inhibitors that alter their activity. Moreover, research on EC 1.17.2 enzymes advances the field of biocatalytic processes. These enzymes can be used in the enzymatic synthesis of complicated molecules and the synthesis of important chemicals in industrial biotechnology.

Our service workflow

EC 1.17.2 enzymes with MCD as a receptor play a crucial role in catalyzing oxidation or reduction reactions involving CH-CH2 groups. Creative Enzymes is dedicated to enzyme activity assays and based on our advanced technology platform, we help researchers, whose enzyme activities can provide valuable insights into their function using spectrophotometric assays. If you are interested in us, please feel free to contact us.