Enzyme Activity Measurement for Arylsulfatase

With years of exploration, Creative Enzymes has become a globally recognized leader in the development of enzymatic analytical services. By constantly striving for services of utmost quality, Creative Enzymes has earned the trust of countless customers and is fully qualified for offering highly sophisticated research services. Herein, Creative Enzymes offers reliable activity measurement for arylsulfatase.

Arylsulfatase (EC 3.1.6.1; aryl-sulfate sulfohydrolase) is a widely distributed enzyme in mammalian, bacterial, fungal and mollusk species, and their primary structures are similar to each other although they originate from different species. There have been several reports of arylsulfatase activity isolated from bacteria, such as Klebsiella, Salmonella, Enterobacter, Serratia, Pseudomonas, and Escherichia coli. Arylsulfatase is a class of glycosulfohydrolase involved in desulfation of sulfated polysaccharides by hydrolyzing arylsulfate ester bond to yield aryl compounds and inorganic sulfate. Arylsulphatases have been classified as type I and II according to their substrate specificity and sensitivity to inhibitors. Type I enzymes are specific for p-nitrophenyl sulphate (pNPS) and p-acetylphenyl phosphate, and are inhibited by cyanide. Type II enzymes are more active on p-nitrocatechol sulphate (pNCS) (2-hydroxy-5-nitrophenyl sulphate), and are inhibited by phosphate and sulphate.

Functionally, arylsulfatase plays a pivotal role in medical research. For instance, arylsulfatase can serve as an enzyme component of anti-tumor antibody-enzyme conjugates for target chemotherapy. Moreover, arylsulfatase is exploited to be used in the diagnosis of metachromatic leukodystrophy. Therefore, to elucidate the catalytic mechanism of arylsulfatase is vital considering its fundamental importance, which also sheds light on its usage of tumor therapy.

Creative Enzymes is proud to provide the most accurate enzyme activity measurement for arylsulfatase. For details, arylsulfatase activity is determined by measuring the amount of p-nitrophenol liberated from p-nitrophenyl sulfate. The amount of p-nitrophenol liberated is determined by measuring the absorbance at 410 nm with the most advanced spectrophotometer. Our results are the most trusted and reliable, resulting in quality services with a high rate of repeat sales. Creative Enzymes is committed to being your best partner who offers the leading technical support and personalized customer services found nowhere else in the industry.

Figure: The crystal structure of arylsulfatase from Pseudomonas aeruginosa.
PDB: 1HDH