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Phosphogluconate dehydrogenase from Human, Recombinant

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Cat No.

NATE-1652

Description

In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+↔ D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.

Abbr

6PGD, Recombinant (Human)

Source

E. coli

Species

Human

Product Overview

This protein is fused with 6x His tag at N terminus and the protein has a calculated MW of 55.3 kDa (503aa).

Form

Liquid

Enzyme Commission Number

EC 1.1.1.44

Activity

>10 units/mg

Molecular Weight

53.3 kDa

Purity

> 90% by SDS-PAGE

Concentration

1 mg/ml

Unit Definition

One unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25°C, in the presence of ß-NADP.

Storage

Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.

Synonyms

6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating

Formulation

1 mg/ml solution in 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA and 10% glycerol.

"6-Phosphogluconic Dehydrogenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0796	6-Phosphogluconate dehydrogenase from E. coli, Recombinant	EC 1.1.1.44	9073-95-4	E. coli	Inquiry
NATE-1937	6-Phosphogluconic Dehydrogenase from Microorganism	EC 1.1.1.44		Microorganism	Inquiry
NATE-0008	Native Sheep 6-Phosphogluconic Dehydrogenase	EC 1.1.1.44	9073-95-4	Sheep liver	Inquiry
NATE-0009	Native Yeast 6-Phosphogluconic Dehydrogenase	EC 1.1.1.44	9073-95-4	Yeast	Inquiry

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