Description
In enzymology, a phosphogluconate dehydrogenase (decarboxylating) (EC 1.1.1.44) is an enzyme that catalyzes the chemical reaction:6-phospho-D-gluconate + NADP+↔ D-ribulose 5-phosphate + CO2 + NADPH. Thus, the two substRates of this enzyme are 6-phospho-D-gluconate and NADP+, whereas its 3 products are D-ribulose 5-phosphate, CO2, and NADPH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Enzyme Commission Number
EC 1.1.1.44
Activity
>40 U/mg protein
Contaminants
(as 6PGDH activity = 100 %)
Glucokinase < 0.01 %
Phosphoglucomutase < 0.01 %
Hexose-6-phosphate isomerase < 0.01 %
Glutathione reductase < 0.01 %
Molecular Weight
ca. 132,000
Isoelectric point
ca. 4.5
Michaelis Constant
(80 mM GIycylglycine buffer, pH 7.5, at 30 °C)
6-Phospho-D-gluconate, 0.95 mM
NAD+, 0.32 mM
Unit Definition
One unit of activity is defined as the amount of 6PGDH that forms 1 μmol of NADH per minute at 30 °C.
Activators
Mg2+, Mn2+, Ca2+, K+, Na+
Thermal stability
(50 mM MES-NaOH buffer, pH 6.8, containing 0.5 M KCl)
No detectable decrease in activity up to 40 °C.
Storage
Stable at -20 °C for at least six months
Stabilizers
KCl, MgCl2, Sorbitol, BSA
Inhibitors
Fructose 1,6-bisphosphate, Erythrose 4-phosphate, NADH
Synonyms
6-Phosphogluconic Dehydrogenase; phosphogluconic acid dehydrogenase; 6-phosphogluconic dehydrogenase; 6-phosphogluconic carboxylase; 6-phosphogluconate dehydrogenase (decarboxylating); 6-phospho-D-gluconate dehydrogenase; EC 1.1.1.44; phosphogluconate dehydrogenase; decarboxylating; 9073-95-4
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