Description
In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2↔ 2 dehydroascorbate + 2 H2O. Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. This enzyme participates in ascorbate metabolism. It employs one cofactor, copper.
Abbr
AAO, Native (Zucchini)
Applications
AAO can be used in clinical tests for determining levels of ascorbic acid in blood or for the removal of interference effects caused by ascorbic acid in clinical analysis.
Appearance
Light tanish, brownish, greyish to blue green free flowing powder
Enzyme Commission Number
EC 1.10.3.3
Contaminants
Adenylate Kinase < 0.5 U/mg; Catalase < 0.096%; Glucose Oxidase < 0.002 U/mg; Cholesterol Oxidase < 0.002 U/mg; Lactate Oxidase < 0.002U/mg; Uricase < 0.002 U/mg
Unit Definition
One unit of activity is defined as the amount of enzyme that will catalyse the oxidation of 1.0 micromole of ascorbic acid per minute at 37°C under the standard assay method conditions. Refer to Table 1 for guidance on factors to adjust units according to temperature of assay.
Thermal stability
Stable at 50°C and below
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate:O2 oxidoreductase; AA oxidase; EC 1.10.3.3; L-ascorbate oxidase
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