Description
In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2↔ 2 dehydroascorbate + 2 H2O. Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. This enzyme participates in ascorbate metabolism. It employs one cofactor, copper.
Abbr
AAO, Native (Acremonium sp.)
Applications
This enzyme is useful for avoidance from interference of ascorbic acid on diagnostic assay such as blood, uric acid, TG, TC and creatinine.
Appearance
Light blue amorphous powder, lyophilized
Enzyme Commission Number
EC 1.10.3.3
Contaminants
Catalase < 0.02%; ATPase < 0.001%
Molecular Weight
80 kDa（gel filtration）
pH Stability
6.0‒10.0（30°C, 24 hr）
Michaelis Constant
Ascorbic acid（pH 7.0） 1.0 × 10-4 M Ascorbic acid（pH 4.0） 3.8 × 10-4 M
Thermal stability
Stable at 50°C and below (pH 7.0, 10 mins)
Storage
Storage at －20°C in the presence of a desiccant is recommended.
Stabilizers
BSA, Mannitol
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate:O2 oxidoreductase; AA oxidase; EC 1.10.3.3; L-ascorbate oxidase
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