Description
In enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction2 L-ascorbate + O2 ↔ 2 dehydroascorbate + 2 H2O. Thus, the two substrates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O.
Abbr
L-ascorbate oxidase (Cucurbita sp.)
Applications
This enzyme is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
Appearance
Light blue amorphous powder, lyophilized
Product Overview
Native L-ascorbate oxidase (EC 1.10.3.3) was purified from Acremonium sp..
Form
Light blue lyophilized powder.
Enzyme Commission Number
EC 1.10.3.3
Contaminants
Catalase < 1.0×10⁻¹ % Phosphatase < 2.0×10⁻² %
pH Stability
pH 6.0-10.0 (25°C, 20hr)
Michaelis Constant
3.0×10-4M(Ascorbate)
Specificity
The enzyme oxidizes ascorbic acid and several ascorbic acid derivatives.
Unit Definition
One unit causes the decrease of one micromole of ascorbic acid per minute under the conditions described below.
Thermal stability
below 45°C (pH 7.0, 30min)
Stability
Stable at-20°C for at least one year
Storage
Store in tightly closed containers, desiccated, protected from light, at-20°C.
Inhibitors
cyanide, Na₂S, diethyldithiocarbamate (Na)
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate: O2 oxidoreductase; AA oxidase; EC 1.10.3.3; 9029-44-1; L-ascorbate oxidase
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