Description
Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate. Take advantage of the improved stability in liquid reagents. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified AAO (Cucurbita sp.)
Applications
Use Ascorbate Oxidase, chemically modified, in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate or creatinine.
Appearance
Turquoise lyophilizate
Activity
>180 U/mg lyophilizate (+37°C, L-ascorbate); Specific activity (+37°C): >1,800 U/mg protein
Contaminants
Catalase: <0.2 Glutamate oxalacetate transaminase (AST): <0.0003 Glutamate pyruvate transaminase (ALT): <0.0005 Contaminating oxidases (FOX): <0.0002
Molecular Weight
Approximately 140 kD
Isoelectric point
5.0-6.0
Michaelis Constant
L-ascorbate: 3 x 10-4 mol/l
Specificity
Several analogs of ascorbate react.
Thermal stability
Up to +70°C
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Keep tightly sealed.
Inhibitors
4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
Synonyms
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate: O2 oxidoreductase; AA oxidase; L-ascorbate oxidase
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