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Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase
Cat No.
NATE-0004
Description
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Abbr
3-HBDH, Native (Rhodopseudomonas sphaeroides)
Alias
3-HBDH
Source
Rhodopseudomonas sphaeroides
Applications
Suitable for the determination of acetoacetate and D (-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Form
Lyophilized powder containing Tris buffer salts
Enzyme Commission Number
EC 1.1.1.30
Activity
250-750 units/mg protein
CAS No.
9028-38-0
Unit Definition
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37°C.
Storage
−20°C
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1099 | Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant | EC 1.1.1.30 | 9028-38-0 | Microorganism | Inquiry |
| NATE-1714 | D-3-hydroxybutyrate dehydrogenase from Microorganism | EC 1.1.1.30 | 9028-38-0 | Microorganism | Inquiry |
| DIA-204 | Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase | EC 1.1.1.30 | 9028-38-0 | Pseudomonas sp. | Inquiry |
| NATE-0003 | Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase | EC 1.1.1.30 | 9028-38-0 | Pseudomonas lemoignei | Inquiry |