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D-3-hydroxybutyrate dehydrogenase from Microorganism

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Activity

Size

Price

Qty

Activity>300 U/mg

Size30 KU

Price$398

Qty

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Cat No.

NATE-1714

Description

In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.

Abbr

HBDH (Microorganism)

Source

Microorganism

Form

Yellowish powder, lyophilized

Enzyme Commission Number

EC 1.1.1.30

Activity

>1500U/mg protein

CAS No.

9028-38-0

Molecular Weight

27.5 kDa (SDS-PAGE)

Isoelectric point

7.25

pH Stability

7.0~10.0 (25℃, 2 hr)

Michaelis Constant

2.1 ×10^-3 M (D-3-Hydroxybutyrate)

Unit Definition

One unit converts one micromole of 3- Hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.

Optimum pH

Optimum temperature

6 ℃

Thermal stability

< 37℃(pH 8.5, 30min)

Storage

Store at -20°C.

Inhibitors

Zn2+, Cu2+, Fe3+

Synonyms

(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30

Notes

INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.

"3-HBDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1099	Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant	EC 1.1.1.30	9028-38-0	Microorganism	Inquiry
DIA-204	Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas sp.	Inquiry
NATE-0003	Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Pseudomonas lemoignei	Inquiry
NATE-0004	Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase	EC 1.1.1.30	9028-38-0	Rhodopseudomonas sphaeroides	Inquiry

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