Description
In enzymology, a 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) is an enzyme that catalyzes the chemical reaction: (R)-3-hydroxybutanoate + NAD+ ↔ acetoacetate + NADH + H+. Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its three products are acetoacetate, NADH, and H+. This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.
Abbr
D-3-hydroxybutyrate dehydrogenase (Pseudomonas sp.)
Applications
This enzyme is useful for enzymatic determination of ketone bodies (D-3-hydroxybutyrate and acetoacetate) in clinical analysis.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.1.30
Activity
GradeⅢ 100U/mg-solid or more
Contaminants
Malate dehydrogenase < 2.0×10⁻³% Lactate dehydrogenase < 2.0×10⁻³% NADH oxidase < 2.0×10⁻³%
Molecular Weight
approx. 130 kDa (by gel filtration)
Isoelectric point
5.6±0.1
pH Stability
pH 5.0-8.5 (25°C, 20hr)
Michaelis Constant
4.2×10⁻⁴M (25°C, pH8.3), 7.0×10⁻⁴M(37°C, pH8.3)(D-3-Hydroxybutyrate) 4.9×10⁻⁵M (25°C, pH8.3), 7.2×10⁻⁵M (37°C, pH8.3)(NAD⁺) 8.1×10⁻⁵M (25°C, pH7.1), 2.4×10⁻⁴M (37°C, pH7.1)(Acetoacetate) 8.4×10⁻⁶M (25°C, pH7.1), 1.5×10⁻⁵M (37°C, pH7.1)(NADH)
Thermal stability
below 40°C (pH 6.5, 15min)
Stability
Stable at-20°C for at least one year
Stabilizers
Sucrose, mannitol, bovine serum albumin
Inhibitors
PCMB, MIA, IAA, Ag⁺, Hg⁺⁺, SDS, DAC
Synonyms
(R)-3-hydroxybutanoate: NAD+ oxidoreductase; NAD+-beta-hydroxybutyrate dehydrogenase; hydroxybutyrate oxidoreductase; beta-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; D-3-hydroxybutyrate dehydrogenase; D-(–)-3-hydroxybutyrate dehydrogenase; beta-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyrate dehydrogenase; beta-hydroxybutyric dehydrogenase; EC 1.1.1.30
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