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Native Pseudomonas lemoignei β-Hydroxybutyrate Dehydrogenase
Cat No.
NATE-0003
Description
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Abbr
3-HBDH, Native (Pseudomonas lemoignei)
Alias
3-HBDH
Source
Pseudomonas lemoignei
Applications
Suitable for the determination of acetoacetate and D (-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
Form
Lyophilized powder containing sucrose, β-NAD and Tris buffer salts
Enzyme Commission Number
EC 1.1.1.30
Activity
> 200 units/mg protein
CAS No.
9028-38-0
Unit Definition
One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37°C.
Storage
−20°C
Synonyms
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 1.1.1.30; 9028-38-0
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1099 | Prokaryotic 3-Hydroxybutyrate dehydrogenase, Recombinant | EC 1.1.1.30 | 9028-38-0 | Microorganism | Inquiry |
| NATE-1714 | D-3-hydroxybutyrate dehydrogenase from Microorganism | EC 1.1.1.30 | 9028-38-0 | Microorganism | Inquiry |
| DIA-204 | Native Pseudomonas sp. D-3-hydroxybutyrate dehydrogenase | EC 1.1.1.30 | 9028-38-0 | Pseudomonas sp. | Inquiry |
| NATE-0004 | Native Rhodopseudomonas sphaeroides β-Hydroxybutyrate Dehydrogenase | EC 1.1.1.30 | 9028-38-0 | Rhodopseudomonas sphaeroides | Inquiry |