Description
PDE3 is a phosphodiesterase. The PDEs belong to at least eleven related gene families, which are different in their primary structure, substrate affinity, responses to effectors, and regulation mechanism. Most of the PDE families are composed of more than one gene. PDE3 is clinically significant because of its role in regulating heart muscle, vascular smooth muscle and platelet aggregation. PDE3 inhibitors have been developed as pharmaceuticals, but their use is limited by arrhythmic effects and they can increase mortality in some applications.
Abbr
PDE, Native (Porcine)
Applications
May be used to assay the protein activator, calmodulin.
Package
Package size based on activated units.
Form
Lyophilized preparation which has been depleted of calmodulin and containing buffer salts as Tris-HCl.
Enzyme Commission Number
EC 3.1.4.17
Molecular Weight
mol wt ~60 kDa
Purity
affinity chromatography
Unit Definition
One unit will hydrolyze 1.0 μmole of 3':5',-cyclic-AMP to 5'-AMP per min at pH 7.5 at 30°C.
Buffer
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0°C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approx. 30% in 24 hrs. if stored at 4°C.
Warnings
Protein determined by Warburg-Christian unless specified otherwise.
Pathway
Insulin signaling pathway, organism-specific biosystem; Insulin signaling pathway, conserved biosystem; Morphine addiction, organism-specific biosystem; Morphine addiction, conserved biosystem; Progesterone-mediated oocyte maturation, organism-specific biosystem; Progesterone-mediated oocyte maturation, conserved biosystem; Purine metabolism, organism-specific biosystem
Synonyms
cyclic 3',5'-mononucleotide phosphodiesterase; PDE; cyclic 3',5'-nucleotide phosphodiesterase; cyclic 3',5'-phosphodiesterase; 3',5'-nucleotide phosphodiesterase; 3':5'-cyclic nucleotide 5'-nucleotidohydrolase; 3',5'-cyclonucleotide phosphodiesterase; cyclic nucleotide phosphodiesterase; 3', 5'-cyclic nucleoside monophosphate phosphodiesterase; 3':5'-monophosphate phosphodiesterase (cyclic CMP); cytidine 3':5'-monophosphate phosphodiesterase (cyclic CMP); cyclic 3',5-nucleotide monophosphate phosphodiesterase; nucleoside 3',5'-cyclic phosphate diesterase; nucleoside-3',5-monophosphate phosphodiesterase; EC 3.1.4.17
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