Description
Hydrolyzes the 3',5'-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5'-monophosphate.
Abbr
PDE, Native (Bovine)
Applications
Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold. For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.
Package
Package size based on activated units
Form
lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
Enzyme Commission Number
EC 3.1.4.17
Activity
>0.1 units/mg protein (with added activator)
Molecular Weight
mol wt ~60 kDa
Unit Definition
One unit will hydrolyze 1.0 μmole of 3':5',-cyclic-AMP to 5'-AMP per min at pH 7.5 at 30°C.
Buffer
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0°C. However, the activator deficient activity may increase up to 200%. If stored at 4°C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.
Warnings
Crude preparation which has been stripped of the protein activator, calmodulin. Protein determined by Warburg-Christian unless specified otherwise.
Function
calcium-and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; protein binding
Synonyms
cyclic 3',5'-mononucleotide phosphodiesterase; PDE; cyclic 3',5'-nucleotide phosphodiesterase; cyclic 3',5'-phosphodiesterase; 3',5'-nucleotide phosphodiesterase; 3':5'-cyclic nucleotide 5'-nucleotidohydrolase; 3',5'-cyclonucleotide phosphodiesterase; cyclic nucleotide phosphodiesterase; 3', 5'-cyclic nucleoside monophosphate phosphodiesterase; 3':5'-monophosphate phosphodiesterase (cyclic CMP); cytidine 3':5'-monophosphate phosphodiesterase (cyclic CMP); cyclic 3',5-nucleotide monophosphate phosphodiesterase; nucleoside 3',5'-cyclic phosphate diesterase; nucleoside-3',5-monophosphate phosphodiesterase; EC 3.1.4.17
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