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Native Crotalus adamanteus venom Phosphodiesterase I

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Cat No.

NATE-0512

Description

Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.

Abbr

PDE I, Native (Crotalus adamanteus venom)

Alias

PDE I; 5'-NPDase; 5'-PDase; 5'-PDE; 5'NPDE

Source

Crotalus adamanteus venom

Form

Lyophilized in vials.

Enzyme Commission Number

EC 3.1.4.1

Activity

> 20 units per mg dry weight

CAS No.

9025-82-5

Unit Definition

One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.

Storage

−20°C

Synonyms

Phosphodiesterase I; EC 3.1.4.1; 5'-exonuclease; 5'-phosphodiesterase; 5'-nucleotide phosphodiesterase; oligonucleate 5'-nucleotidohydrolase; 5' nucleotide phosphodiesterase/alkaline phosphodiesterase I; 5'-NPDase; 5'-PDase; 5'-PDE; 5'NPDE; alkaline phosphodiesterase; nucleotide pyrophosphatase/phosphodiesterase I; orthophosphoric diester phosphohydrolase; PDE I; phosphodiesterase; exonuclease I

"PDE" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0511	Native Bothrops atrox Phosphodiesterase I	EC 3.1.4.1	9025-82-5	Bothrops atrox	Inquiry
NATE-0513	Native Crotalus atrox (Western Diamondback Rattlesnake) Phosphodiesterase I	EC 3.1.4.1	9025-82-5	Crotalus atrox (Western Diamondback Rattlesnake)	Inquiry
NATE-0514	Native Bovine Phosphodiesterase 3',5'-Cyclic Nucleotide Activator-deficient	EC 3.1.4.17	9040-59-9	Bovine heart	Inquiry
NATE-0515	Native Bovine Phosphodiesterase, 3',5'-cyclic-nucleotide-specific	EC 3.1.4.17	9040-59-9	Bovine brain	Inquiry

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