Description
Hydrolyzes the 3',5'-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5'-monophosphate.
Abbr
PDE, Native (Bovine)
Applications
May be used to assay the protein activator, calmodulin.
Package
Package size based on activated units
Form
Lyophilized powder containing Tris-HCl buffer salts and lactose
Enzyme Commission Number
EC 3.1.4.17
Activity
15-30 units/mg protein (in the presence of 0.03 mM Ca2+ and a saturating level (10 units per ml) of calmodulin (P2277))
Molecular Weight
mol wt ~60 kDa
Unit Definition
One unit will hydrolyze 1.0 μmole of 3',5'-cyclic-AMP to 5'-AMP per min at pH 7.5 at 30°C.
Buffer
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20°C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4°C.
Warnings
Affinity purified preparation which has been depleted of calmodulin. Protein determined by Warburg-Christian
Pathway
Ca-dependent events, organism-specific biosystem; calmodulin binding; protein binding
Synonyms
cyclic 3',5'-mononucleotide phosphodiesterase; PDE; cyclic 3',5'-nucleotide phosphodiesterase; cyclic 3',5'-phosphodiesterase; 3',5'-nucleotide phosphodiesterase; 3':5'-cyclic nucleotide 5'-nucleotidohydrolase; 3',5'-cyclonucleotide phosphodiesterase; cyclic nucleotide phosphodiesterase; 3', 5'-cyclic nucleoside monophosphate phosphodiesterase; 3':5'-monophosphate phosphodiesterase (cyclic CMP); cytidine 3':5'-monophosphate phosphodiesterase (cyclic CMP); cyclic 3',5-nucleotide monophosphate phosphodiesterase; nucleoside 3',5'-cyclic phosphate diesterase; nucleoside-3',5-monophosphate phosphodiesterase; EC 3.1.4.17
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