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Native Microorganism Creatine Amidinohydrolase

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Activity

Size

Price

Qty

Activity4 U/mg

Size20 KU

Price$525

Qty

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Cat No.

DIA-185

Description

Creatine Amidinohydrolase catalyzes the hydrolytic reaction converting creatine to sarcosine and urea. The enzyme is purified from a microorganism. The molecular weight of the enzyme is approximately 67,000. The enzyme is useful for the enzymatic assay of creatine and creatinine when coupled with other related enzymes. creatine + H2O → sarcosine + urea

Abbr

Creatinase, Native (Microorganism)

Alias

Creatinase

Source

Microorganism

Applications

This enzyme is useful for enzymatic determination of creatinine when coupled with creatinine amidohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Appearance

White amorphous powder, lyophilized

Form

Freeze dried powder

Enzyme Commission Number

EC 3.5.3.3

Activity

GradeⅡ 4.0 U/mg-solid or more

CAS No.

37340-58-2

Contaminants

NADH oxidase < 5.0×10⁻²%; Catalase < 2.0%

Molecular Weight

approx. 67 kDa (by gel filtration)

Isoelectric point

4.5±0.1

pH Stability

pH 4.0-10.0 (25°C, 20hr)

Michaelis Constant

4.5×10－3 M (Creatine)

Structure

2 subunits per mol of enzyme

Optimum pH

6.5-7.5

Optimum temperature

40－50°C

Thermal stability

below 50°C (pH 7.5, 30min)

Stability

Stable at -20°C for at least one year

Stabilizers

Sugars, EDTA

Inhibitors

Hg⁺⁺, Cu⁺⁺, Ag⁺, SH reagent (NEM), PCMB

Synonyms

Creatine Amidinohydrolase; Creatinase; EC 3.5.3.3

"Creatinase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0160	Native Actinobacillus sp. Creatinase	EC 3.5.3.3	37340-58-2	Actinobacillus sp.	Inquiry
NATE-0161	Native Flavobacterium sp. Creatinase	EC 3.5.3.3	37340-58-2	Flavobacterium sp.	Inquiry
NATE-0162	Creatinase from Pseudomonas sp., Recombinant	EC 3.5.3.3	37340-58-2	E. coli	Inquiry
NATE-1241	Creatinase from E. coli, Recombinant	EC 3.5.3.3	37340-58-2	E. coli	Inquiry

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