Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Native (Actinobacillus sp.)
Source
Actinobacillus sp.
Applications
Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.
Form
Lyophilized powder containing sugars and EDTA as stabilizers
Enzyme Commission Number
EC 3.5.3.3
Activity
20-40 units/mg protein
Molecular Weight
mol wt ~100 kDa
Isoelectric point
4.6 ± 0.1
pH Stability
pH 5.5 − 9.0 (25°C, 16hr)
Michaelis Constant
1.9 x 10‾2M (Creatine)
Structure
2 subunits per mole of enzyme
Unit Definition
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37°C.
Thermal stability
Below 50°C (pH 7.5, 30 min)
Inhibitors
Cu++, Hg++, Ag+
Synonyms
Creatine amidinohydrolase; creatinase; 37340-58-2; EC 3.5.3.3
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