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Creatinase from Pseudomonas sp., Recombinant
Cat No.
NATE-0162
Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Recombinant (Pseudomonas sp.)
Alias
creatinase
Source
E. coli
Species
Pseudomonas sp.
Applications
Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.
Product Overview
recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein
Form
lyophilized powder
Enzyme Commission Number
EC 3.5.3.3
Activity
10-20 units/mg protein
CAS No.
37340-58-2
Composition
Protein, ~70% biuret
Unit Definition
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37°C.
Stability
2-8°C
Synonyms
Creatine amidinohydrolase; creatinase; 37340-58-2; EC 3.5.3.3
Download
Download Datasheet"Creatinase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-185 | Native Microorganism Creatine Amidinohydrolase | EC 3.5.3.3 | 37340-58-2 | Microorganism | Inquiry |
| NATE-0160 | Native Actinobacillus sp. Creatinase | EC 3.5.3.3 | 37340-58-2 | Actinobacillus sp. | Inquiry |
| NATE-0161 | Native Flavobacterium sp. Creatinase | EC 3.5.3.3 | 37340-58-2 | Flavobacterium sp. | Inquiry |
| NATE-1241 | Creatinase from E. coli, Recombinant | EC 3.5.3.3 | 37340-58-2 | E. coli | Inquiry |