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Creatinase from E. coli, Recombinant
Cat No.
NATE-1241
Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O↔ sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Appearance
White lyophilizate
Enzyme Commission Number
EC 3.5.3.3
Activity
≥ 15 U/mg
CAS No.
37340-58-2
Contaminants
catalase < 0.5%
Molecular Weight
ca. 80 kDa
pH Stability
4.0–11.0
Michaelis Constant
8.6 x 10^-3 M (creatine)
Structure
2 subunits of 48 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of urea per min at 37°C and pH 7.7.
Optimum pH
7.0–9.0
Optimum temperature
45°C
Thermal stability
below 53°C
Storage
at -20°C
Stabilizers
Sucrose
Inhibitors
Hg2+
Synonyms
Creatine amidohydrolase; Creatinase; EC 3.5.3.3
Download
Download Datasheet"Creatinase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-185 | Native Microorganism Creatine Amidinohydrolase | EC 3.5.3.3 | 37340-58-2 | Microorganism | Inquiry |
| NATE-0160 | Native Actinobacillus sp. Creatinase | EC 3.5.3.3 | 37340-58-2 | Actinobacillus sp. | Inquiry |
| NATE-0161 | Native Flavobacterium sp. Creatinase | EC 3.5.3.3 | 37340-58-2 | Flavobacterium sp. | Inquiry |
| NATE-0162 | Creatinase from Pseudomonas sp., Recombinant | EC 3.5.3.3 | 37340-58-2 | E. coli | Inquiry |