Description
Neuraminidase enzymes are glycoside hydrolase enzymes (EC 3.2.1.18) that cleave the glycosidic linkages of neuraminic acids. Neuraminidase enzymes are a large family, found in a range of organisms. The best-known neuraminidase is the viral neuraminidase, a drug target for the prevention of the spread of influenza infection. The viral neuraminidases are frequently used as antigenic determinants found on the surface of the Influenza virus. Some variants of the influenza neuraminidase confer more virulence to the virus than others. Other homologs are found in mammalian cells, which have a range of functions. At least four mammalian sialidase homologs have been described in the human genome (see NEU1, NEU2, NEU3, NEU4).
Abbr
Neuraminidase, Native (Vibrio cholerae)
Applications
Neurminidase is used as a cell-surface probe for glyc oconjugate distribution and in substrate specificity studies. Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.
Product Overview
Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.
Form
Type I, buffered aqueous solution; Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2; Type II, buffered aqueous solution, Solution in 50 mM sodium acetate, pH 5.5, containing 0.15 M sodium chloride and 4 mM calcium chloride; Type III, sterile filtered, aqueous solution, pH 5.5, 0.15 M NaCl and 4 mM CaCl2.
Enzyme Commission Number
EC 3.2.1.18
Activity
Type I, 1-5 units/mg protein (Lowry, using NAN-lactose); Type II, 8-24 units/mg protein (Lowry, using NAN-lactose); Type III, > 1.5 units/mg protein.
Unit Definition
One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37°C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.
Warnings
Chromatographically purified.
Pathway
Other glycan degradation, organism-specific biosystem (from KEGG) Other glycan degradation, conserved biosystem (from KEGG) Vibrio cholerae infection, organism-specific biosystem (from KEGG) Vibrio cholerae infection, conserved biosystem (from KEGG)
Synonyms
neuraminidase; sialidase; α-neuraminidase; acetylneuraminidase; exo-α-sialidase; EC 3.2.1.18; 9001-67-6
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