Description
Sphingomyelin phosphodiesterase is a hydrolase enzyme that is involved in sphingolipid metabolism reactions. SMase is a member of the DNase I superfamily of enzymes and is responsible for breaking sphingomyelin (SM) down into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stresses.
Abbr
SMase, Native (Streptomyces sp.)
Applications
This enzyme is useful for enzymatic determination of sphingomyelin when coupled With alkaline phosphatase and choline oxidase.
Appearance
White to brownish amorphous powder，lyophilized
Enzyme Commission Number
EC 3.1.4.12
Molecular Weight
37.5 kDa (SDS‒PAGE)
Michaelis Constant
Sphingomyelin 0.45 × 10‒3M
Unit Definition
One unit is defined as the amount of enzyme which hydrolyzes 1 μmole of sphingomyelin per minute at 37°C under the conditions specified in the assay procedure.
Activators
Mg2+, Mn2+, Non‒ionic detergents
Thermal stability
Stable at 40°C and below
Stability
At least one year at －20°C
Storage
Storage at －20°C in the presence of a desiccant is recommended.
Synonyms
Sphingomyelin phosphodiesterase; EC 3.1.4.12; neutral sphingomyelinase; 9031-54-3; sphingomyelin cholinephosphohydrolase; sphingomyelinase; Smase
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