Description
Alpha-glucosidase is a glucosidase located in the brush border of the small intestine that acts upon 1,4-alpha bonds. This is in contrast to beta-glucosidase. Alpha-glucosidase breaks down starch and disaccharides to glucose. Maltase, a similar enzyme that cleaves maltose, is nearly functionally equivalent.
Abbr
α-Glucosidase, Native (Saccharomyces cerevisiae)
Source
Saccharomyces cerevisiae
Applications
For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.
Product Overview
Protein determined by biuret.
Enzyme Commission Number
EC 3.2.1.20
Activity
4-8 units/mg; > 10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
Molecular Weight
Mr ~63 kDa
Unit Definition
One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37°C.
Pathway
Amino acid transport across the plasma membrane, organism-specific biosystem; Galactose metabolism, conserved biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Starch and sucrose metabolism, conserved biosystem; Transmembrane transport of small molecules, organism-specific biosystem
Function
alpha-1,4-glucosidase activity; cation binding; maltose alpha-glucosidase activity; hydrolase activity; maltose alpha-glucosidase activity
Synonyms
α-glucosidase; maltase; glucoinvertase; glucosidosucrase; maltase-glucoamylase; α-glucopyranosidase; glucosidoinvertase; α-D-glucosidase; α-glucoside hydrolase; α-1,4-glucosidase; EC 3.2.1.20; 9001-42-7
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