Description
Creatinine Amidohydrolase catalyzes the hydrolytic reaction converting creatinine to creatine. The enzyme is purified from a microorganism. The molecular size of the enzyme is approximately 175,000. The enzyme is useful for the enzy-matic assay of creatinine when coupled with other related enzymes. Creatinine + H2O → Creatine.
Abbr
Creatinine amidohydrolase (Pseudomonas sp.)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Product Overview
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Form
Lyophilized powder containing sucrose and BSA as stabilizers
Enzyme Commission Number
EC 3.5.2.10
Activity
> 250U/mg protein
pH Stability
pH 7.5-9.0 (5°C, 16hr)
Michaelis Constant
3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure
6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Unit Definition
One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C
Thermal stability
Below 70°C (pH 7.5, 30 min)
Inhibitors
Ag+, Hg++, N-bromosuccinimide, EDTA
Function
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides.
Synonyms
creatininase; creatinine hydrolase; creatinine amidohydrolase; EC 3.5.2.10; 9025-13-2
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