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Creatininase from E. coli, Recombinant

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Cat No.

NATE-1242

Description

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

Abbr

Creatininase, Recombinant (E. coli)

Source

E. coli

Species

E. coli

Appearance

White lyophilizate

Enzyme Commission Number

EC 3.5.2.10

Activity

> 500 U/mg lyophilizate

CAS No.

9025-13-2

Contaminants

catalase < 1.0%

Molecular Weight

ca. 170 kDa

Isoelectric point

4.8

pH Stability

7.0–11.0

Michaelis Constant

3.4 x 10^-2 M (creatinine) 4.3 x 10^-2 M (creatine)

Structure

6 subunits of 28 kDa (SDS-PAGE)

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of creatine per min at 37°C and pH 6.8.

Optimum pH

6.5–7.0

Optimum temperature

60–65°C

Activators

Mg2+, Mn2+

Thermal stability

below 60°C

Storage

at -20°C

Inhibitors

Hg2+

Synonyms

EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2

"Creatininase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-130	Native Pseudomonas sp. Creatinine amidohydrolase	EC 3.5.2.10	9025-13-2	Pseudomonas sp.	Inquiry
NATE-0163	Native microorganisms Creatininase	EC 3.5.2.10	9025-13-2	microorganisms	Inquiry
EXWM-4493	creatininase	EC 3.5.2.10	9025-13-2		Inquiry