Description
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Abbr
Creatininase, Native (microorganisms)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Product Overview
Protein determined by biuret.
Form
Lyophilized powder containing sucrose and BSA as stabilizers
Enzyme Commission Number
EC 3.5.2.10
Activity
100-300 units/mg protein
Molecular Weight
mol wt ~175 kDa
pH Stability
pH 7.5 − 9.0 (5°C, 16hr)
Michaelis Constant
3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine) Structure: 6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Unit Definition
One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25°C
Thermal stability
Below 70°C (pH 7.5, 30 min)
Inhibitors
Ag+, Hg++, N-bromosuccinimide, EDTA
Synonyms
EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2
Download Datasheet