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Native Pseudomonas sp. Cholesterol Oxidase

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Cat No.

DIA-136

Description

Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.

Abbr

Cholesterol Oxidase (Pseudomonas sp.)

Source

Pseudomonas sp.

Appearance

Yellowish amorphous powder, lyophilized

Product Overview

Recombinant Cholesterol Oxidase produced in E.coli has a molecular mass of about 34,000 Da.

Enzyme Commission Number

EC 1.1.3.6

Activity

Grade Ⅲ 15 U/mg-solid or more(contg. approx. 40% of stabilizers)

CAS No.

9026-00-0

Contaminants

Catalase < 1.0%; Cholesterol esterase < 1.0×10-2%

Molecular Weight

about 34 kDa (gel filtration)

Isoelectric point

5.1±0.1 and 5.4±0.1

pH Stability

pH 5.0~10.0 (25°C, 20hr)

Michaelis Constant

2.1×10-2 M (Creatine)

Structure

dimer

Optimum pH

6.5~7.0

Optimum temperature

45~50°C

Thermal stability

below 45°C (pH 7.0, 15min)

Stability

Stable at-20°C for at least 6 months

Inhibitors

onic detergents, Hg2＋, Ag

Synonyms

Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6

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Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-138	Native Microorganism Cholesterol Oxidase	EC 1.1.3.6	9028-76-6	Microorganism	Inquiry
DIA-284	Chemically modified Cholesterol Oxidase from Brevibacterium species			Brevibacterium species	Inquiry
DIA-406	Cholesterol oxidase from E. coli, Recombinant	EC 1.1.3.6	9028-76-6	E. coli	Inquiry
DIA-408	Cholesterol oxidase from E. coli, Recombinant (liquid)	EC 1.1.3.6	9028-76-6	E. coli	Inquiry

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