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Cholesterol oxidase from E. coli, Recombinant

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Cat No.

DIA-406

Description

Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.

Abbr

CHOD, Recombinant (E. coli)

Alias

CHOD

Source

E. coli

Species

E. coli

Appearance

Yellow lyophilizate

Enzyme Commission Number

EC 1.1.3.6

Activity

> 5 U/mg lyophilizate

CAS No.

9028-76-6

Contaminants

catalase < 2.0% cholesterol esterase < 0.02%

Molecular Weight

ca. 58 kDa

pH Stability

5.0–10.0

Michaelis Constant

3.5 x 10^-4 M (cholesterol)

Structure

monomer of 55 kDa (SDS-PAGE)

Unit Definition

One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.0.

Optimum pH

Optimum temperature

50°C

Thermal stability

below 55°C

Inhibitors

ionic detergents, Ag+, Hg2+

Synonyms

EC 1.1.3.6, cholesterol-O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; 9028-76-6

"CHOD" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-136	Native Pseudomonas sp. Cholesterol Oxidase	EC 1.1.3.6	9026-00-0	Pseudomonas sp.	Inquiry
DIA-138	Native Microorganism Cholesterol Oxidase	EC 1.1.3.6	9028-76-6	Microorganism	Inquiry
DIA-284	Chemically modified Cholesterol Oxidase from Brevibacterium species			Brevibacterium species	Inquiry
DIA-408	Cholesterol oxidase from E. coli, Recombinant (liquid)	EC 1.1.3.6	9028-76-6	E. coli	Inquiry

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