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Native Microorganism Cholesterol Oxidase
Cat No.
DIA-138
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Oxidase (Microorganism)
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol esterase in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.6
Activity
GradeIII 12U/mg-solid or more
CAS No.
9028-76-6
Contaminants
Catalase < 1.0×10⁻¹% Cholesterol esterase < 1.0×10⁻²%
Molecular Weight
approx. 55 kDa (by gel-filtration)
pH Stability
pH 5.0-10.0 (25°C, 20hr)
Michaelis Constant
3.0×10⁻⁵M(Cholesterol)
Optimum pH
7.0-8.0
Optimum temperature
60°C
Thermal stability
below 60°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least 9 months
Stabilizers
Bovine serum albumin, amino acids
Inhibitors
Ionic detergents, Hg⁺⁺
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-136 | Native Pseudomonas sp. Cholesterol Oxidase | EC 1.1.3.6 | 9026-00-0 | Pseudomonas sp. | Inquiry |
| DIA-284 | Chemically modified Cholesterol Oxidase from Brevibacterium species | Brevibacterium species | Inquiry | ||
| DIA-406 | Cholesterol oxidase from E. coli, Recombinant | EC 1.1.3.6 | 9028-76-6 | E. coli | Inquiry |
| DIA-408 | Cholesterol oxidase from E. coli, Recombinant (liquid) | EC 1.1.3.6 | 9028-76-6 | E. coli | Inquiry |