Description
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7. Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.
Abbr
APase, Native (Potato)
Applications
Phosphatase acid from potato has been used in a study to develop a method of efficient enzymatic hydrolysis of polyprenyl pyrophosphates. It has also been used in a study to investigate the kinetics of the hydrolysis of sodium p-nitrophenylphosphate and other phosphoric acid monoesters.
Product Overview
Acid phosphatase from potatoes is a 111 kDa diner consisting of two subunits at 41 and 35 kDa. This phosphatase has also been shown to cleave DNA.
Form
Type I, Type IV, ammonium sulfate suspension; Suspension in 1.8 M (NH4)2SO4, 10 mM MgCl2, pH 5.5; Type II, Type III, lyophilized powder.
Enzyme Commission Number
EC 3.1.3.2
Activity
Type I, > 200 units/mg protein (biuret); Type II, 0.5-3.0 unit/mg solid; Type III, 3.0-10.0 units/mg solid; Type IV, > 10.0 units/mg protein (modified Warburg-Christian).
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37°C.
Synonyms
acid phosphatase; 9001-77-8; acid phosphomonoesterase; phosphomonoesterase; glycerophosphatase; acid monophosphatase; acid phosphohydrolase; acid phosphomonoester hydrolase; uteroferrin; acid nucleoside diphosphate phosphatase; orthophosphoric-monoester phosphohydrolase (acid optimum); EC 3.1.3.2; APase
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