Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction: sn-glycerol 3-phosphate + O2 ↔ glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
L-α-glycerophosphate oxidase (Pediococcus sp.)
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.21
Activity
GradeⅢ 40 U/mg-solid or more (containing approx. 40% of stabilizers)
Contaminants
Lactate oxidase < 1.0×10⁻³%
Molecular Weight
approx. 76 kDa (by gel filtration)
Isoelectric point
4.1±0.1
pH Stability
pH 6.5-8.5 (25°C, 20hr)
Michaelis Constant
3.2×10⁻³M (L-α-Glycerophosphate), 6.8×10⁻³M (D, L-form)
Specificity
The enzyme has the highest specificity for L-form of α-glycerophosphate.
Optimum temperature
35-40°C
Thermal stability
below 40°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least one year (A decrease in activity of ca. 10% may occur at 5°C within 6 months.)
Inhibitors
Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺
Synonyms
L-α-glycerophosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; EC 1.1.3.21
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