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Glycerol-3-phosphate Oxidase from E. coli, Recombinant

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Cat No.

DIA-286

Description

Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Rely on the proven diagnostic quality of this product.

Abbr

GPO, Recombinant (E. coli)

Alias

GPO

Source

E. coli

Applications

Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.

Appearance

Greenish yellow lyophilizate

Activity

>90 U/mg lyophilizate (+37°C)

CAS No.

9046-28-0

Contaminants

Cholesterol oxidase: <0.001 Lactate oxidase: <0.002 Uricase: <0.001

Molecular Weight

75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)

Isoelectric point

~4.2

pH Stability

6.5-8.5

Michaelis Constant

K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)

Specificity

Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.

Optimum pH

8.0-8.5

Stability

At +2 to +8°C within specification range for 12 months. Store dry.

Inhibitors

Ag, Hg-salts and SDS

Synonyms

glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO

"Glycerol-3-phosphate oxidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-154	Native Pediococcus sp. Glycerol-3-phosphate oxidase	EC 1.1.3.21	9046-28-0	Pediococcus sp.	Inquiry
DIA-199	Native Pediococcus sp. L-α-glycerophosphate oxidase	EC 1.1.3.21	9046-28-0	Pediococcus sp.	Inquiry
DIA-200	Native Microorganism L-α-glycerophosphate oxidase	EC 1.1.3.21	9046-28-0	Microorganism	Inquiry
DIA-287	Chemically modified Glycerol-3-phosphate Oxidase from E. coli			E. coli	Inquiry

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