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Chemically modified Glycerol-3-phosphate Oxidase from E. coli

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Cat No.

DIA-287

Description

Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Take advantage of the enhanced liquid stability of this enzyme. Rely on the proven diagnostic quality of this product.

Abbr

Chemically modified GPO, Recombinant (E. coli)

Alias

GPO

Source

E. coli

Applications

Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.

Appearance

Green yellow amorphous lyophilizate

Activity

>10 U/mg lyophilizate (+37°C, L-α-glycerol phosphate); Specific activity (+25°C): >40 U/mg protein

Contaminants

Cholesterol oxidase: <0.001 Lactate oxidase: <0.002 Uricase: <0.05

Molecular Weight

75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)

Isoelectric point

~4.2

pH Stability

6.5-8.5

Michaelis Constant

K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)

Specificity

Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.

Optimum pH

8.0-8.5

Stability

At +2 to +8°C within specification range for 12 months. Store dry.

Inhibitors

Ag, Hg-salts and SDS

Synonyms

glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO

"Glycerol-3-phosphate oxidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-154	Native Pediococcus sp. Glycerol-3-phosphate oxidase	EC 1.1.3.21	9046-28-0	Pediococcus sp.	Inquiry
DIA-199	Native Pediococcus sp. L-α-glycerophosphate oxidase	EC 1.1.3.21	9046-28-0	Pediococcus sp.	Inquiry
DIA-200	Native Microorganism L-α-glycerophosphate oxidase	EC 1.1.3.21	9046-28-0	Microorganism	Inquiry
DIA-286	Glycerol-3-phosphate Oxidase from E. coli, Recombinant		9046-28-0	E. coli	Inquiry

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