Description
In enzymology, a glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the chemical reaction:sn-glycerol 3-phosphate + O2↔ glycerone phosphate + H2O2. Thus, the two substrates of this enzyme are sn-glycerol 3-phosphate and O2, whereas its two products are glycerone phosphate and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycerophospholipid metabolism. It employs one cofactor, FAD.
Abbr
GPO, Native (Pediococcus sp.)
Applications
This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase and glycerokinase in clinical analysis.
Form
Lyophilized powder containing stabilizers
Enzyme Commission Number
EC 1.1.3.21
Activity
40-80 units/mg solid
Molecular Weight
~76 kDa (gel filtration)
Isoelectric point
4.1-/+0.1
pH Stability
6.5-8.5 (25°C, 20hr)
Michaelis Constant
3.2x10-3M (L-α-Glycerophosphate), 6.8 x 10-3M (D, L-form)
Unit Definition
One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H2O2 per min at 37°C, at the appropriate pH.
Thermal stability
below 40°C (pH 7.0, 15min)
Inhibitors
Ionic detergents (SDS, LBS, etc.), Hg++, Ag+
Synonyms
EC 1.1.3.21; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-α-glycerophosphate oxidase; α-glycerophosphate oxidase; L-α-glycerol-3-phosphate oxidase; Glycerol 3-phosphate Oxidase; 9046-28-0; sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase; L-Glycerol 3-phosphate Oxidase; GPO
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