Description
Xanthine oxidase is a form of xanthine oxidoreductase, a type of enzyme that generates reactive oxygen species. These enzymes catalyze the oxidation of hypoxanthine to xanthine and can further catalyze the oxidation of xanthine to uric acid. These enzymes play an important role in the catabolism of purines in some species, including humans.
Applications
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5'-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase and uricase.
Appearance
Reddish brown amorphous powder, lyophilized
Enzyme Commission Number
EC 1.1.3.22
Activity
GradeⅡ 10U/mg-solid or more
Contaminants
Catalase < 5% Adenosine deaminase < 1.0×10⁻³% Uricase < 1.0×10⁻³% Phosphatase < 1.0×10⁻³% Purine-nucleoside phosphorylase < 5.0×10⁻³%
Molecular Weight
approx. 160 kDa
Isoelectric point
4.0±0.1
pH Stability
pH 6.5-9.0 (25°C, 15hr)
Michaelis Constant
4.5×10⁻⁵M (Xanthine), 7.6×10⁻⁵M (Hypoxanthine)
Thermal stability
below 55°C (pH 8.0, 30min)
Stability
Stable at-20°C for at least year
Stabilizers
Sodium glutamate, BSA
Inhibitors
Reducing agents, Hg⁺⁺, Ag⁺,MIA
Synonyms
EC 1.1.3.22; Xanthine oxidase; XO; XAO
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